Study of hypoglycemic effects and antioxidant potential of polyherbal formulation in rats
نویسندگان
چکیده
Present study was designed to evaluate the hypoglycemic effect and antioxidant potential of polyherbal formulation in streptozotocin (STZ) induced diabetic rats. Five different groups were made including 10 rats in each group. An aqueous suspension of herbal mixture containing 5 ingredients of equal amounts– leaves of Azadirachta indica (Margosa), leaves of Gymnema sylvestre (Gurmar), fruits of Momordica charantia (Bitter gourd), seeds of Syzigium cumini (Black plum) and seeds of Trigonella foenum (Fenugreek) was administered orally once a day at a dose of 500 mg/kg body weight for a period of 4 weeks. In these groups lipid peroxidation (LPO), reduced glutathione (GSH) , glutathione S -transferase (GST), proteins, urea & cholesterol was estimated in liver and kidney as bioindicators of oxidative stress. The antidiabetic effect of herbal formulation was compared with antidiabetic drug ‘Glibenclamide’. Our observations showed that herbal treatment restored the elevated blood glucose level to normal range in diabetic rats. The formulation also prevented the diabetes-induced decrease in the body weight of rats. Results showed an increased level of GSH and GST and significant decline in LPO with herbal treatment in the tissues of normal and diabetic animals, while no significant change was observed in GSH & GST level with glibenclamide treated diabetic group. Level of serum urea increased to almost two fold in diabetic rats as compared with the values observed in the control group. The urea level restored to normal range in diabetic rats after receiving the herbal mixture. Serum cholesterol was significantly lower in diabetic rats as compared to control. The combination of these five herbal drugs appeared more effective in low dose as an antidiabetic and antioxidant agent than the individual herbal drug, when used alone. This herbal formulation also appeared to be devoid of any significant toxic effect in animals.
منابع مشابه
Hypoglycemic and Antioxidant Effects of a Polyherbal Formulation in Alloxan Induced Diabetic Rats
Objective: The present study was conducted to determine the hypoglycemic and antioxidant potential of polyherbal formulation in alloxan induced diabetic rats. Study Design: In five different groups an aqueous suspension of herbal mixture containing equal amounts of– Azadirachta indica, Gymnema sylvestre, Momordica charantia, Syzigium cumini, Trigonella foenum was administered orally once a day ...
متن کاملAntidiabetic activity of a polyherbal formulation (DRF/AY/5001).
The herbal formulation, DRF/AY/5001, elicits hypoglycemic/antidiabetic effects in both normal and experimentally induced hyperglycemic (epinephrine and alloxan) rats. Further, herbal formulation treatment can significantly alter the pattern of glucose tolerance in normal and diabetic rats. It is possible that the herbal formulation may act through both, pancreatic and extra-pancreatic mechanism...
متن کاملStudy of hypoglycemic, hypocholesterolemic and antioxidant activities of Iranian Mentha spicata leaves aqueous extract in diabetic rats
This study was conducted to evaluate the hypoglycemic, hypocholesterolemic and antioxidant properties of Mentha spicata (Labiateae) leaves aqueous extract (MSLA) in alloxan-induced diabetic rats. In this study, hyperglycemia was induced in male rats by intraperitoneal injection of alloxan monohydrate (150 mg/kg). The aqueous extract of M. spicata was orally administered at a dose of 300 mg/kg b...
متن کاملStudy of hypoglycemic, hypocholesterolemic and antioxidant activities of Iranian Mentha spicata leaves aqueous extract in diabetic rats
This study was conducted to evaluate the hypoglycemic, hypocholesterolemic and antioxidant properties of Mentha spicata (Labiateae) leaves aqueous extract (MSLA) in alloxan-induced diabetic rats. In this study, hyperglycemia was induced in male rats by intraperitoneal injection of alloxan monohydrate (150 mg/kg). The aqueous extract of M. spicata was orally administered at a dose of 300 mg/kg b...
متن کاملImproved Glucoregulation, Insulin Resistance and Leptin Levels by a Polyherbal Drug in High Fat Diet and Low Dose Streptozotocin Type 2 Diabetes Model
The aim of the present study was to evaluate the antidiabetic and lipid lowering potential of a polyherbal formulation of six different Indian medicinal plants. Animals were divided into groups as follows: (a) normal control; (b) high fat diet control; (c) high fat diet + streptozotocin (STZ) control; (d) high fat diet + STZ + polyherbal formulation; and (e) high fat diet + STZ + rosiglitazone....
متن کامل